Return to calling text

The protein titration needs to span ~5 orders of magnitude for the specific reason of obtaining good estimates of the limits L and U.

at low concentrations, fractional protection approaches a lower limit L

at high concentrations, fractional protection approaches an upper limit U

the linear relationship P = (U-L)*Y  + L is used to convert fractional protection (which runs from L to U) to fractional occupancy (Y, which runs from 0 to 1).

It is the fractional occupancy isotherm (ie, data that is bounded by 0 and 1) that we usually see reported, as this form of the data allows comparison between different experiments and testing goodness of fit to modeled curves.

The fortran module that is to be described here incorporates this function so that the raw data, fractional protection, is modeled as a function of fractional occupancy, which is itself modeled in terms of protein concentration and free energy constants for intrinsic binding and cooperativity interactions.

Return to calling text