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My students and I are involved in four areas of photosynthesis
research.
Structure/Function Studies of Photosystem
I in Cyanobacteria
The first area involves largely biophysical techniques, and includes
study of the structure, function and organization of the Photosystem
I reaction center complex in cyanobacteria and plants. Our goal
is to discover how reaction centers convert light into chemical
free energy with a quantum efficiency of nearly 100%.
Bioassembly of Photosystem I in Cyanobacteria
and Plants
The second area involves largely molecular biological techniques,
and includes study of the genes, proteins, and factors involved
in the assembly of iron-sulfur clusters, particularly those which
participate in Photosystem I. Our goal is to discover how its bioassembly
is regulated, and focusses on the regulation of the Suf and Isc
systems of iron-sulfur cluster assembly.
Type I Reaction Centers in Photosynthetic
Anaerobes
The third area involves largely biochemical techniques, and includes
study of structure, function and organization the photosynthetic
reaction centers in the anaerobic heliobacteria and green sulfur
bacteria. Our goal is to uncover the protein and cofactor composition
of photosynthetic reaction centers in these primitive anaerobic
organisms.
Coupling Photosystem I to hydrogenase
The fourth area involves the fabrication and study of an engineered
biological/organic hybrid half-cell that couples Photosystem I,
which efficiently captures and stores the energy derived from sunlight,
with a hydrogenase, which generates high rates of dihydrogen with
the input of Gibbs free energy. The challenge is to deliver the
low-potential electron from Photosystem I to the hydrogenase in
vitro using a method that does not depend on inefficient solution
(diffusion) chemistry.
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