ࡱ> /1.@ pjbjb ddp lfffffff  [^" , VFfF\ff\\\:ff\zffff\\ff ]@[[/\/\BMB 474 Analytical Techniques in Biochemistry and Molecular Biology (2 credits) Theory and practice of analytical chemistry applied to proteins and nucleic acids. Topics: chromatography, electrophoresis, centrifugation, spectroscopy and X-ray crystallography. Prerequisite: HYPERLINK "http://www.psu.edu/bulletins/bluebook/courses/b_m_b/428.htm"BMB 428 or CHEM 451 (may be taken concurrently) The revised title more accurately reflects the nature of the course content. Course Description: This two-credit course deals with acquiring the laboratory skills required for success in experimental biochemistry and molecular biology. It is particularly suited for students intending a career as a technician or research scientist in the areas of biochemistry, biotechnology, bioengineering, microbiology, or molecular biology. The course content focuses on the detection, purification and identification of biological macromolecules such as proteins and nucleic acids. The material is divided into two equal parts. The first part includes the theory and practice of analytical biochemistry with emphasis on diffusion, gel permeation chromatography, ion-exchange chromatography, affinity chromatography, sedimentation velocity ultracentrifugation, sedimentation equilibrium ultracentrifugation, density gradient ultracentrifugation, agarose gel electrophoresis, SDS gel electrophoresis and isoelectrofocusing of proteins and nucleic acids. The second part includes the theory and practice of biological spectroscopy with emphasis on visible, infrared, circular dichroism, optical rotary dispersion, resonance Raman, nuclear magnetic resonance and electron paramagnetic resonance spectroscopy of proteins and nucleic acids. A final two lectures are devoted to the theory and practice of X-ray crystallography of macromolecules. The lectures are designed to introduce a particular topic, to derive the relevant equations, to supplement the reading material with practical examples, and to clarify points in the assigned problem sets. Two guest lectures by experts in the field provide up-to-date information on mass spectroscopy and nuclear magnetic resonance spectroscopy, and two field-trips, one to an analytical ultracentrifugation laboratory and the other to an electron paramagnetic resonance laboratory provide hands-on experience. Problem sets are assigned at the beginning of each class, and these should be completed prior to the next period. The problem sets are not collected or graded; rather, the answers are handed out in the following class period. This method provides the ability to collaborate with others on solving problems and to self-check work. The midterm examination tests material in first part of the course on analytical biochemistry and the final examination tests material in the second part of the course on biological spectroscopy. The final examination is therefore not cumulative. Each examination consists of variations of the problem sets along with material from the book and the lectures. The final grade is 50% midterm examination, 50% final examination. The instructor is available in two scheduled weekly office hours or by appointment.  E]^_ghkta . $%noop 5B*ph B*PJphjPJU jPJU B*ph5PJPJRop L!] 5Lp@ P !$`'0*-/@&Rop!"p/ =!"#$%DyK yK xhttp://www.psu.edu/bulletins/bluebook/courses/b_m_b/428.htm i<@< NormalCJOJPJQJmH sH tH <A@< Default Paragraph FontnBn Body TextB 5Lp@ P !$`'0*-/]CJPJJY@J Document Map-D M OJPJQJp  zp p p p ^gp X _Hlt491696710_r @ar 8?q& r Rr : Linda Kunes@Lulu's HD:Users:lindakunes:Desktop:BMB 474 Analytical Techn.doco r @[p @ @GTimes New Roman5Symbol3 Arial3Times;Helvetica71-3 0000 ph F FA >0d p Q@BMB 474 Analytical Techniques in Biochemistry and Molecular Biology (2) Theory and application of analytical techniques with e John Golbeck Linda Kunes Oh+'0$<HX lx   'BMB 474 Analytical Techniques in Biochemistry and Molecular Biology (2) Theory and application of analytical techniques with eMB  John GolbeckytiohnNormall Linda Kunes2ndMicrosoft Word 10.1@F#@|L&@|L&A  ՜.+,D՜.+,h hp|  H'PSU  BMB 474 Analytical Techniques in Biochemistry and Molecular Biology (2) Theory and application of analytical techniques with e Title 8@ _PID_HLINKS'Aj<http://www.psu.edu/bulletins/bluebook/courses/b_m_b/428.htm  !"#$%'()*+,-0Root Entry Fdq 2Data 1TableWordDocumentSummaryInformation(DocumentSummaryInformation8&CompObjXObjectPooldq dq  FMicrosoft Word DocumentNB6WWord.Document.8