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Publications by Ming Tien

2007

Ross, D. E., S. S. Ruebush, S. L. Brantley, R. S. Hartshorne, T. A. Clarke, D. J. Richardson, and M. Tien. Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 73:5797-5808.

Sato, S., F. Liu, H. Koc, and M. Tien. Expression analysis of extracellular proteins from Phanerochaete chrysosporium grown on different liquid and solid substrates. Microbiology 153: 3023-3033.

Liu, F., H. Koc, and M. Tien. Proteomic analysis of extracellular enzymes produced by wood-degrading fungi. In: Manual of Environmental Microbiology, 3rd ed. (Ronald L. Crawford, ed), pp. 1089-1096, ASM Press, Washington D.C.

2006

Romero, H.M., E.J. Pell, and M. Tien. Expression profile analysis and biochemical properties of the peptide methionine sulfoxide reductase A (PMSRA) gene family in Arabidopsis. Plant Sci. 170:705-714.

Ruebush, S.S., S.L. Brantley, and M. Tien. Reduction of soluble and insoluble iron forms by membrane fractions of Shewanella oneidensis grown under aerobic and anaerobic conditions. Appl. Environ. Microb. 72:2925-2935.

Ruebush, S.S., G.A. Icopini, S.L. Brantley, and M. Tien. In vitro enzymatic reduction kinetics of mineral oxides by membrane fractions from Shewanella oneidensis MR-1. Geochim. Cosmochim. Acta 70:56-70.

Brantley, S.L., S. Ruebush, J-H Jang, and M. Tien. Analysis of (bio)geochemical kinetics of Fe(III) oxides. In: Methods of Investigating Microbial-Mineral Interactions, CMS Workshop Lectures. (J. P. A. Maurice and L. A. Warren, eds.), vol. 14, pp. 79-116, The Clay Mineral Society, Chantilly, VA.

2005

Abbas, A., H. Koc, F. Liu, and M. Tien. Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate. Curr. Genet. 47:49-56.

2004

Icopini, G.A., A.D. Anbar, S.S. Ruebush, M. Tien, and S.L. Brantley. Iron isotope fractionation during microbial reduction of iron: The importance of adsorption. Geology 32:205-208.

Romero, H.M., B.S. Berlett, P.J. Jensen, E.J. Pell, and M. Tien. Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis. Plant Physiol. 136:3784-3794.

2003

Banci, L., I. Bartalesi, S. Ciofi-Baffoni, and M. Tien. Unfolding and pH studies on manganese peroxidase: Role of heme and calcium on secondary structure stability. Biopolymers 72:38-47.

Varela, E. and M. Tien. Effect of pH and oxalate on hydroquinone-derived hydroxyl radical formation during brown rot wood degradation. Appl. Envir. Microbiol. 69:6025-6031.

Varela, E., T. Mester, and M. Tien. Culture conditions affecting biodegradation components of the brown-rot fungus Gloeophyllum trabeum. Arch. Microbiol. 180:251-256.

2001

Mester, T., and M. Tien. Engineering of a manganese-binding site in lignin peroxidase isozyme H8 from Phanerochaete chrysosporium. Biochem. Bioph. Res. Comm. 284:723-728.

Mester, T., and M. Tien. Oxidation mechanism of ligninolytic enzymes involved in the degradation of environmental pollutants. Inter. Biodet. Biodeg. 46:51-59.

Cheng, H., T. Tchaikovskaya, Y.-S.L. Tu, J. Chapman, B. Qian, W.-M. Ching, M. Tien, J.D. Rowe, Y.V. Patskovsky, I. Listowsky, and C.-P.D. Tu. Rat glutathione S-transferase M4-4: An isoenzyme with unique structural features including a redox-reactive cysteine-115 residue that forms mixed disulphides with glutathione. Biochem. J. 356:403-414.

Mester, T., K. Ambert-Balay, S. Ciofi-Baffoni, L. Banci, A.D. Jones, and M. Tien. Oxidation of a tetrameric nonphenolic lignin model compound by lignin peroxidase. J. Biol. Chem. 276:22985-22990.

2000

Santucci, R., C. Bongiovanni, S. Marini, R. Del Conte, M. Tien, L. Banci, and M. Coletta. Redox Equilibria of Manganese Peroxidase from Phanerochaetes Chrysosporium: Functional Role of Residues on the Proximal Side of the Haem Pocket. Biochem. J. 349:85-90.

Ambert-Balay, K., M. Dougherty, and M. Tien. Reactivity of Manganese Peroxidase: Site-directed Mutagenesis of Residues in Proximity to the Porphyrin Ring. Arch. Biochem. Biophys. 382:89-94.

Mester, T. and M. Tien. Oxidation Mechanism of Ligninolytic Enzymes Involved in the Degradation of Environmental Pollutants Intern. Biodet. Biodegrad. 46:51-59.

1999

Banci, L., I. Bertini, C. Capannoli, R. Del Conte, and M. Tien. Spectroscopic Characterization of Active Mutants of Manganese Peroxidase: Mutations on the Proximal Side Affect Calcium Binding of the Distal Side. Biochemistry 38:9617-9625.

Banci, L., S. Ciofi-Baffoni, and M. Tien. Lignin and Mn Peroxidase-catalyzed Oxidation of Phenolic Lignin Oligomers. Biochemisty 38:3205-3210.

Tien, M. Myeloperoxidase-catalyzed Oxidation of Tyrosine. Arch. Biochem. Biophys. 367:61-66.

Tien, M., B.S. Berlett, R.L. Levine, P.B. Chock, and E.R. Stadtman. Peroxynitrite-mediated Modification of Proteins at Physiological Carbon Dioxide Concentration: pH Dependence of Carbonyl Formation, Tyrosine Nitration, and Methionine Oxidation. Proc. Natl. Acad. Sci. USA 96:7809-7814.

1998

Ambert-Balay, K., S.M. Fuchs, and M. Tien. Identification of the Veratryl Alcohol Binding Site in Lignin Peroxidase by Site-directed Mutagenesis. Biochem. Biophys. Res. Commun. 251:283-286.

Banci, L., I. Bertini, L. dal Pozzo, R. del Conte, and M Tien. Monitoring the Role of Oxalate in Manganese Peroxidase. Biochemistry 37:9009-9015.

Zapanta, L.S., T. Hattori, M. Rzetskaya, and M. Tien. The Cloning of Phanerochaete chrysosporium leu2 by Complementation of Bacterial Auxotrophs and Transformation of Fungal Auxotrophs. Appl. Environ. Microbiol. 64:2624-2629.

1997

Janse, B.J.H., J. Gaskell, D. Cullen, L. Zapanta, M.J. Dougherty, and M. Tien. Are Bacteria Omnipresent on Phanerochaete chrysosporium Burdsall? Appl. Environ. Microbiol. 63:2913-2914.

Tien, M. and D. Ma. Oxidation of 4-Methoxymandelic Acid by Lignin Peroxidase. J. Biol. Chem. 272:8912-8917.

Whitwam, R.E., K.R. Brown, M. Musick, M.J. Natan, and M. Tien. Mutagenesis of the Mn²⁺-Binding Site of Manganese Peroxidase Affects Oxidation of Mn²⁺ by both Compound I and Compound II. Biochemistry 36:9766-9773.

1996

Stewart, P., R.E. Whitwam, P.J. Kersten, D. Cullen, and M. Tien. Efficient Expression of a Phanerochaete chrysosporium Manganese Peroxidase Gene in Aspergillus oryzae. Appl. Environ. Microbiol. 6:860-864.

Koduri, R.S., R.E. Whitwam, D. Barr, S.D. Aust, and M. Tien. Oxidation of 1,2,4,5-Tetramethoxybenzene by Lignin Peroxidase of Phanerochaete chrysoporium. Arch. Biochem. Biophys. 326:216-265.

Nanny, M.A., J.M. Bortiatynski, M. Tien, and P.G. Hatcher. Investigations of Enzymatic Alterations of 2,4-dichlorophenol Using 13C-Nuclear Magnetic Resonance in Combination with Site-Specific 13C-Labeling: Understanding the Environmental Fate of This Pollutant. Environ. Toxicol. Chem. 15:1857-1864.

Whitwam, R. and M. Tien. Heterologous Expression and Reconstitution of Fungal Mn Peroxidase. Arch. Biochem. Biophys. 333:439-446.

Myer, S. and M. Tien. Characterization of Biodegradation Catalyzed by Mutant Strains of Phanerochaete Chrysosporium and other Fungi. In: Culture Collector to Improve the Quality of Life. (R.A. Samson, J.A. Stalpers, D. van der Mei, and A.H. Stouthamer, eds.), pp. 344-351, Ponsen & Looyen, Wageningen, Netherlands.

1995

Banci, L., I. Bertini, R. Pierattelli, M. Tien, and A.J. Vila. Factoring of the Hyperfine Shifts in the Cyanide Adduct of Lignin Peroxidase from Phanerochaete Chrysosporium. J. Am. Chem. Soc. ll7:8659-8667.

Koduri, R.S. and M. Tien. Oxidation of Guaiacol by Lignin Peroxidase: Role of Veratryl Alcohol. J. Biol. Chem. 270:22254-22258.

Orth, A.B., M. Rzhetskaya, E.A. Pell, and M. Tien. A Ser(Thr) Protein Kinase Confers Fungicide Resistance in the Phytopathogenic Fungus Ustilago maydis. Appl. Environ. Microbiol. 6l:234l-2345.

Orth, A.B. and M. Tien. Biotechnology of Lignin Degradation (Invited Review). In: The Mycota II Genetics and Biotechnology. (Kuch, ed.), pp. 287-302. Springer-Verlag, Berlin, Heidelberg.

Whitwam, R.E., I.G. Gazarian, and M. Tien. Expression of Fungal Mn Peroxidase in E. coli and Refolding to Yield Active Enzyme. Biochem. Biophys. Res. Commun. 2l6:l0l3-l0l7.

1994

Koduri, R.S. and M. Tien. Kinetic Analysis of Lignin Peroxidase: Explanation for the Mediation Phenomena by Veratryl Alcohol. Biochemistry 33:4225-4230.

Orth, A.B., E.A. Pease, and M. Tien. Properties of Lignin-Degrading Peroxidase and their Use in Bioremediation. In: Biological Degradation and Bioremediation of Toxic Chemicals. (G.R. Chaudhry, ed.), p. 403-410. Chapman & Hall, New York, NY.

Orth, A.B., M. Rzhetskaya, D. Cullen, and M. Tien. Characterization of cDNA Encoding a Manganese Peroxidase from Phanerochaete chrysoporium: Genomic Organization of Lignin and Manganese Peroxidase Genes. Gene 148:161-165.

Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. Characterization and Genetic Analysis of Laboratory Mutants of Ustilago maydis Resistant to Dicarboximide and Aromatic Hydrocarbon Fungicides. Phytopathology 84:l2l0-l2l4.

Wedler, F., R. Bernlohr, R. Hardison, T.-h. Kao, and M. Tien. Solutions Manual for Principles of Biochemistry (2nd edition). (Textbook by Lehninger, Nelson, and Cox), Worth Publishers, Inc., New York, NY.

1993

Banci, L., I. Bertini, I.-c. Kuan, M. Tien, P. Turano, and A.J. Vila. NMR Investigation of Isotopically Labeled Cyanide Derivatives of Lignin Peroxidase and Manganese Peroxidase. Biochemistry 32:13483-13489.

Banci, L., I. Bertini, T. Bini, M. Tien, and P. Turano. Binding of Horseradish, Lignin and Manganese Peroxidase to Their Respective Substrates. Biochemistry 32:5825-583l.

Cai, D. and M. Tien. Lignin-Degrading Peroxidases of Phanerochaete chrysosporium. J. Biotech. 30:79-90.

Cancel, A.M., A.B. Orth, and M. Tien. Lignin and Veratryl Alcohol are not Inducers of the Ligninolytic System of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 59:2909-29l3.

Hammel, K.E., K.A. Jensen, M.D. Mozuch, L.L. Landucci, M. Tien, and E.A. Pease. Ligninolysis by a Purified Lignin Peroxidase. J. Biol. Chem. 268:12274-12281.

Kuan, I.-c. and M. Tien. Stimulation of Mn Peroxidase Activity: A Possible Role for Oxalate in Lignin Biodegradation. Proc. Natl. Acad. Sci. USA 90:1242-1246.

Kuan, I.-c., K.. Johnson, and M. Tien. Kinetic Analysis of Manganese Peroxidase. J. Biol. Chem. 268:20064-20070.

Kuan, I.-c. and M. Tien. Glyoxylate-Supported Reactions Catalyzed by Mn Peroxidase of Phanerochaete chrysosporium: Activity in the Absence of Added Hydrogen Peroxide. Arch. Biochem. Biophys. 302:447-454.

Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. Assessing the Involvement of Free Radicals in Fungicide Toxicity Using a-Tocophereol Analogs Pestic. Biochem. Physiol. 47:134-141.

Orth, A.B., D.J. Royse, and M. Tien. Ubiquity of Lignin-Degrading Peroxidases Among Various Wood-Degrading Fungi. Appl. Environ. Microbiol. 59:40l7-4023.

1992

Johnson, T.M., E.A. Pease, K.-K. Li, and M. Tien. Production and Characterization of Recombinant Lignin Peroxidase Isozyme H2 rom Phanerochaete chrysosporium Using Recombinant Baculovirus. Arch. Biochem. Biophys. 296:660-666.

Pease, E.A. and M. Tien. Heterogeneity and Regulation of Manganese Peroxidase from Phanerochaete chrysosporium. J. Bacteriol. 174:3532-3540.

Cai, D. and M. Tien. Kinetic Studies on the Formation and Decomposition of Compounds II and III: Reactions of Lignin Peroxidase with H2O2. J. Biol. Chem. 267:11149 11155.

Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. An Investigation into the Mode of Action of Aromatic Hydrocarbon and Dicarboximide Fungicides. Pestic. Biochem. Physiol. 44:91-100.

Banci, L., I. Bertini, P.Turano, E.A. Pease, and M. Tien. 1H NMR Investigation of Manganese Peroxidase from Phanerochaete chrysosporium: A Comparison with Other Peroxidases. Biochemistry 31:10009-10017.

Kuan, I.-C. and M. Tien. Stimulation of Mn Peroxidase Activity: A Possible Role for Oxalate in Lignin Biodegradation. Proc. Nat. Acad. Sci. USA 90:1242-1246.

1991

Banci, L., I. Bertini, P. Turano, M. Tien, and T.K. Kirk. Proton NMR Investigation into the Basis for the Relatively High Redox Potential of Lignin Peroxidase. Proc. Nat. Acad. Sci. USA 88:6956-6960.

Cai, D. and M. Tien. Lignin Peroxidase: Catalysis, Oxycomplex, and Heme-Linked Ionization. In: Enzymes in Biomass Conversion. American Chemical Society Series 460 (Leatham and Himmel, eds.), pp 180-187. American Chemical Society, Washington, D.C.

Cai, D. and M. Tien. Lignin Peroxidase of Phanerochaete chrysosporium: Evidence for an Acidic Ionization Controlling Activity. J. Biol. Chem. 266:14464-14469.

Kuan, I.-C., Cai, D., and M. Tien. The Lignin-Degrading System of the Wood-Destroying Fungus Phanerochaete Chrysosporium. In: Active Oxygen/Oxidative Stress and Plant Metabolism. (Pell and Steffen, eds.), pp 180-192. ASPP Publishers, Rockville, MD.

Orth, A.B., M. Denny, and M. Tien. Overproduction of Lignin-Degrading Enzymes by an Isolate of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 57:2591-2596.

Pease, E.A., S.D. Aust, and M. Tien. Heterologous Expression of Active Manganese Peroxidase from Phanerochaete chrysosporium Using the Baculovirus Expression System. Biochem. Biophys. Res. Commun. 179:897-903.


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About Us Faculty Facilities Seminars Publications News and Events Outreach Links	Publications by Ming Tien 2007 Ross, D. E., S. S. Ruebush, S. L. Brantley, R. S. Hartshorne, T. A. Clarke, D. J. Richardson, and M. Tien. Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 73:5797-5808. Sato, S., F. Liu, H. Koc, and M. Tien. Expression analysis of extracellular proteins from Phanerochaete chrysosporium grown on different liquid and solid substrates. Microbiology 153: 3023-3033. Liu, F., H. Koc, and M. Tien. Proteomic analysis of extracellular enzymes produced by wood-degrading fungi. In: Manual of Environmental Microbiology, 3rd ed. (Ronald L. Crawford, ed), pp. 1089-1096, ASM Press, Washington D.C. 2006 Romero, H.M., E.J. Pell, and M. Tien. Expression profile analysis and biochemical properties of the peptide methionine sulfoxide reductase A (PMSRA) gene family in Arabidopsis. Plant Sci. 170:705-714. Ruebush, S.S., S.L. Brantley, and M. Tien. Reduction of soluble and insoluble iron forms by membrane fractions of Shewanella oneidensis grown under aerobic and anaerobic conditions. Appl. Environ. Microb. 72:2925-2935. Ruebush, S.S., G.A. Icopini, S.L. Brantley, and M. Tien. In vitro enzymatic reduction kinetics of mineral oxides by membrane fractions from Shewanella oneidensis MR-1. Geochim. Cosmochim. Acta 70:56-70. Brantley, S.L., S. Ruebush, J-H Jang, and M. Tien. Analysis of (bio)geochemical kinetics of Fe(III) oxides. In: Methods of Investigating Microbial-Mineral Interactions, CMS Workshop Lectures. (J. P. A. Maurice and L. A. Warren, eds.), vol. 14, pp. 79-116, The Clay Mineral Society, Chantilly, VA. 2005 Abbas, A., H. Koc, F. Liu, and M. Tien. Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak substrate. Curr. Genet. 47:49-56. 2004 Icopini, G.A., A.D. Anbar, S.S. Ruebush, M. Tien, and S.L. Brantley. Iron isotope fractionation during microbial reduction of iron: The importance of adsorption. Geology 32:205-208. Romero, H.M., B.S. Berlett, P.J. Jensen, E.J. Pell, and M. Tien. Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis. Plant Physiol. 136:3784-3794. 2003 Banci, L., I. Bartalesi, S. Ciofi-Baffoni, and M. Tien. Unfolding and pH studies on manganese peroxidase: Role of heme and calcium on secondary structure stability. Biopolymers 72:38-47. Varela, E. and M. Tien. Effect of pH and oxalate on hydroquinone-derived hydroxyl radical formation during brown rot wood degradation. Appl. Envir. Microbiol. 69:6025-6031. Varela, E., T. Mester, and M. Tien. Culture conditions affecting biodegradation components of the brown-rot fungus Gloeophyllum trabeum. Arch. Microbiol. 180:251-256. 2001 Mester, T., and M. Tien. Engineering of a manganese-binding site in lignin peroxidase isozyme H8 from Phanerochaete chrysosporium. Biochem. Bioph. Res. Comm. 284:723-728. Mester, T., and M. Tien. Oxidation mechanism of ligninolytic enzymes involved in the degradation of environmental pollutants. Inter. Biodet. Biodeg. 46:51-59. Cheng, H., T. Tchaikovskaya, Y.-S.L. Tu, J. Chapman, B. Qian, W.-M. Ching, M. Tien, J.D. Rowe, Y.V. Patskovsky, I. Listowsky, and C.-P.D. Tu. Rat glutathione S-transferase M4-4: An isoenzyme with unique structural features including a redox-reactive cysteine-115 residue that forms mixed disulphides with glutathione. Biochem. J. 356:403-414. Mester, T., K. Ambert-Balay, S. Ciofi-Baffoni, L. Banci, A.D. Jones, and M. Tien. Oxidation of a tetrameric nonphenolic lignin model compound by lignin peroxidase. J. Biol. Chem. 276:22985-22990. 2000 Santucci, R., C. Bongiovanni, S. Marini, R. Del Conte, M. Tien, L. Banci, and M. Coletta. Redox Equilibria of Manganese Peroxidase from Phanerochaetes Chrysosporium: Functional Role of Residues on the Proximal Side of the Haem Pocket. Biochem. J. 349:85-90. Ambert-Balay, K., M. Dougherty, and M. Tien. Reactivity of Manganese Peroxidase: Site-directed Mutagenesis of Residues in Proximity to the Porphyrin Ring. Arch. Biochem. Biophys. 382:89-94. Mester, T. and M. Tien. Oxidation Mechanism of Ligninolytic Enzymes Involved in the Degradation of Environmental Pollutants Intern. Biodet. Biodegrad. 46:51-59. 1999 Banci, L., I. Bertini, C. Capannoli, R. Del Conte, and M. Tien. Spectroscopic Characterization of Active Mutants of Manganese Peroxidase: Mutations on the Proximal Side Affect Calcium Binding of the Distal Side. Biochemistry 38:9617-9625. Banci, L., S. Ciofi-Baffoni, and M. Tien. Lignin and Mn Peroxidase-catalyzed Oxidation of Phenolic Lignin Oligomers. Biochemisty 38:3205-3210. Tien, M. Myeloperoxidase-catalyzed Oxidation of Tyrosine. Arch. Biochem. Biophys. 367:61-66. Tien, M., B.S. Berlett, R.L. Levine, P.B. Chock, and E.R. Stadtman. Peroxynitrite-mediated Modification of Proteins at Physiological Carbon Dioxide Concentration: pH Dependence of Carbonyl Formation, Tyrosine Nitration, and Methionine Oxidation. Proc. Natl. Acad. Sci. USA 96:7809-7814. 1998 Ambert-Balay, K., S.M. Fuchs, and M. Tien. Identification of the Veratryl Alcohol Binding Site in Lignin Peroxidase by Site-directed Mutagenesis. Biochem. Biophys. Res. Commun. 251:283-286. Banci, L., I. Bertini, L. dal Pozzo, R. del Conte, and M Tien. Monitoring the Role of Oxalate in Manganese Peroxidase. Biochemistry 37:9009-9015. Zapanta, L.S., T. Hattori, M. Rzetskaya, and M. Tien. The Cloning of Phanerochaete chrysosporium leu2 by Complementation of Bacterial Auxotrophs and Transformation of Fungal Auxotrophs. Appl. Environ. Microbiol. 64:2624-2629. 1997 Janse, B.J.H., J. Gaskell, D. Cullen, L. Zapanta, M.J. Dougherty, and M. Tien. Are Bacteria Omnipresent on Phanerochaete chrysosporium Burdsall? Appl. Environ. Microbiol. 63:2913-2914. Tien, M. and D. Ma. Oxidation of 4-Methoxymandelic Acid by Lignin Peroxidase. J. Biol. Chem. 272:8912-8917. Whitwam, R.E., K.R. Brown, M. Musick, M.J. Natan, and M. Tien. Mutagenesis of the Mn²⁺-Binding Site of Manganese Peroxidase Affects Oxidation of Mn²⁺ by both Compound I and Compound II. Biochemistry 36:9766-9773. 1996 Stewart, P., R.E. Whitwam, P.J. Kersten, D. Cullen, and M. Tien. Efficient Expression of a Phanerochaete chrysosporium Manganese Peroxidase Gene in Aspergillus oryzae. Appl. Environ. Microbiol. 6:860-864. Koduri, R.S., R.E. Whitwam, D. Barr, S.D. Aust, and M. Tien. Oxidation of 1,2,4,5-Tetramethoxybenzene by Lignin Peroxidase of Phanerochaete chrysoporium. Arch. Biochem. Biophys. 326:216-265. Nanny, M.A., J.M. Bortiatynski, M. Tien, and P.G. Hatcher. Investigations of Enzymatic Alterations of 2,4-dichlorophenol Using 13C-Nuclear Magnetic Resonance in Combination with Site-Specific 13C-Labeling: Understanding the Environmental Fate of This Pollutant. Environ. Toxicol. Chem. 15:1857-1864. Whitwam, R. and M. Tien. Heterologous Expression and Reconstitution of Fungal Mn Peroxidase. Arch. Biochem. Biophys. 333:439-446. Myer, S. and M. Tien. Characterization of Biodegradation Catalyzed by Mutant Strains of Phanerochaete Chrysosporium and other Fungi. In: Culture Collector to Improve the Quality of Life. (R.A. Samson, J.A. Stalpers, D. van der Mei, and A.H. Stouthamer, eds.), pp. 344-351, Ponsen & Looyen, Wageningen, Netherlands. 1995 Banci, L., I. Bertini, R. Pierattelli, M. Tien, and A.J. Vila. Factoring of the Hyperfine Shifts in the Cyanide Adduct of Lignin Peroxidase from Phanerochaete Chrysosporium. J. Am. Chem. Soc. ll7:8659-8667. Koduri, R.S. and M. Tien. Oxidation of Guaiacol by Lignin Peroxidase: Role of Veratryl Alcohol. J. Biol. Chem. 270:22254-22258. Orth, A.B., M. Rzhetskaya, E.A. Pell, and M. Tien. A Ser(Thr) Protein Kinase Confers Fungicide Resistance in the Phytopathogenic Fungus Ustilago maydis. Appl. Environ. Microbiol. 6l:234l-2345. Orth, A.B. and M. Tien. Biotechnology of Lignin Degradation (Invited Review). In: The Mycota II Genetics and Biotechnology. (Kuch, ed.), pp. 287-302. Springer-Verlag, Berlin, Heidelberg. Whitwam, R.E., I.G. Gazarian, and M. Tien. Expression of Fungal Mn Peroxidase in E. coli and Refolding to Yield Active Enzyme. Biochem. Biophys. Res. Commun. 2l6:l0l3-l0l7. 1994 Koduri, R.S. and M. Tien. Kinetic Analysis of Lignin Peroxidase: Explanation for the Mediation Phenomena by Veratryl Alcohol. Biochemistry 33:4225-4230. Orth, A.B., E.A. Pease, and M. Tien. Properties of Lignin-Degrading Peroxidase and their Use in Bioremediation. In: Biological Degradation and Bioremediation of Toxic Chemicals. (G.R. Chaudhry, ed.), p. 403-410. Chapman & Hall, New York, NY. Orth, A.B., M. Rzhetskaya, D. Cullen, and M. Tien. Characterization of cDNA Encoding a Manganese Peroxidase from Phanerochaete chrysoporium: Genomic Organization of Lignin and Manganese Peroxidase Genes. Gene 148:161-165. Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. Characterization and Genetic Analysis of Laboratory Mutants of Ustilago maydis Resistant to Dicarboximide and Aromatic Hydrocarbon Fungicides. Phytopathology 84:l2l0-l2l4. Wedler, F., R. Bernlohr, R. Hardison, T.-h. Kao, and M. Tien. Solutions Manual for Principles of Biochemistry (2nd edition). (Textbook by Lehninger, Nelson, and Cox), Worth Publishers, Inc., New York, NY. 1993 Banci, L., I. Bertini, I.-c. Kuan, M. Tien, P. Turano, and A.J. Vila. NMR Investigation of Isotopically Labeled Cyanide Derivatives of Lignin Peroxidase and Manganese Peroxidase. Biochemistry 32:13483-13489. Banci, L., I. Bertini, T. Bini, M. Tien, and P. Turano. Binding of Horseradish, Lignin and Manganese Peroxidase to Their Respective Substrates. Biochemistry 32:5825-583l. Cai, D. and M. Tien. Lignin-Degrading Peroxidases of Phanerochaete chrysosporium. J. Biotech. 30:79-90. Cancel, A.M., A.B. Orth, and M. Tien. Lignin and Veratryl Alcohol are not Inducers of the Ligninolytic System of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 59:2909-29l3. Hammel, K.E., K.A. Jensen, M.D. Mozuch, L.L. Landucci, M. Tien, and E.A. Pease. Ligninolysis by a Purified Lignin Peroxidase. J. Biol. Chem. 268:12274-12281. Kuan, I.-c. and M. Tien. Stimulation of Mn Peroxidase Activity: A Possible Role for Oxalate in Lignin Biodegradation. Proc. Natl. Acad. Sci. USA 90:1242-1246. Kuan, I.-c., K.. Johnson, and M. Tien. Kinetic Analysis of Manganese Peroxidase. J. Biol. Chem. 268:20064-20070. Kuan, I.-c. and M. Tien. Glyoxylate-Supported Reactions Catalyzed by Mn Peroxidase of Phanerochaete chrysosporium: Activity in the Absence of Added Hydrogen Peroxide. Arch. Biochem. Biophys. 302:447-454. Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. Assessing the Involvement of Free Radicals in Fungicide Toxicity Using a-Tocophereol Analogs Pestic. Biochem. Physiol. 47:134-141. Orth, A.B., D.J. Royse, and M. Tien. Ubiquity of Lignin-Degrading Peroxidases Among Various Wood-Degrading Fungi. Appl. Environ. Microbiol. 59:40l7-4023. 1992 Johnson, T.M., E.A. Pease, K.-K. Li, and M. Tien. Production and Characterization of Recombinant Lignin Peroxidase Isozyme H2 rom Phanerochaete chrysosporium Using Recombinant Baculovirus. Arch. Biochem. Biophys. 296:660-666. Pease, E.A. and M. Tien. Heterogeneity and Regulation of Manganese Peroxidase from Phanerochaete chrysosporium. J. Bacteriol. 174:3532-3540. Cai, D. and M. Tien. Kinetic Studies on the Formation and Decomposition of Compounds II and III: Reactions of Lignin Peroxidase with H2O2. J. Biol. Chem. 267:11149 11155. Orth, A.B., A. Sfarra, E.J. Pell, and M. Tien. An Investigation into the Mode of Action of Aromatic Hydrocarbon and Dicarboximide Fungicides. Pestic. Biochem. Physiol. 44:91-100. Banci, L., I. Bertini, P.Turano, E.A. Pease, and M. Tien. 1H NMR Investigation of Manganese Peroxidase from Phanerochaete chrysosporium: A Comparison with Other Peroxidases. Biochemistry 31:10009-10017. Kuan, I.-C. and M. Tien. Stimulation of Mn Peroxidase Activity: A Possible Role for Oxalate in Lignin Biodegradation. Proc. Nat. Acad. Sci. USA 90:1242-1246. 1991 Banci, L., I. Bertini, P. Turano, M. Tien, and T.K. Kirk. Proton NMR Investigation into the Basis for the Relatively High Redox Potential of Lignin Peroxidase. Proc. Nat. Acad. Sci. USA 88:6956-6960. Cai, D. and M. Tien. Lignin Peroxidase: Catalysis, Oxycomplex, and Heme-Linked Ionization. In: Enzymes in Biomass Conversion. American Chemical Society Series 460 (Leatham and Himmel, eds.), pp 180-187. American Chemical Society, Washington, D.C. Cai, D. and M. Tien. Lignin Peroxidase of Phanerochaete chrysosporium: Evidence for an Acidic Ionization Controlling Activity. J. Biol. Chem. 266:14464-14469. Kuan, I.-C., Cai, D., and M. Tien. The Lignin-Degrading System of the Wood-Destroying Fungus Phanerochaete Chrysosporium. In: Active Oxygen/Oxidative Stress and Plant Metabolism. (Pell and Steffen, eds.), pp 180-192. ASPP Publishers, Rockville, MD. Orth, A.B., M. Denny, and M. Tien. Overproduction of Lignin-Degrading Enzymes by an Isolate of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 57:2591-2596. Pease, E.A., S.D. Aust, and M. Tien. Heterologous Expression of Active Manganese Peroxidase from Phanerochaete chrysosporium Using the Baculovirus Expression System. Biochem. Biophys. Res. Commun. 179:897-903.
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