Publications by B. Tracy Nixon

2007

Chen, B., M. Doucleff, D. E. Wemmer, S. De Carlo, H. H. Huang, E. Nogales, T. R. Hoover, E. Kondrashkina, L. Guo, and B. T. Nixon.   ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, s54. Structure 15:429-440.

Doucleff, M., J. G. Pelton, P. S. Lee, B. T. Nixon, and D. E. Wemmer.  Structural basis of DNA recognition by the alternative sigma-factor, sigma54.  J. Mol. Biol. 369:1070-1078.

2006

De Carlo, S., B. Chen, T.R. Hoover, E. Kondrashkina, E. Nogales, and B.T. Nixon. The structural basis for assembly and function of the transcriptional activator NtrC. Genes & Dev. 20:1485-1495.

2005

Nixon, B.T., H. P. Yennawar, M. Doucleff, J. G. Pelton, D. E. Wemmer, S. Krueger, and E. Kondrashkina. SAS solution structures of the Apo and Mg(2+)/BeF(3)(-)-bound receiver domain and DctD from Sinorhizobium meliloti. Biochemistry 44:13962-13969.
 
Doucleff, M., B. Chen, A. Maris, D. E. Wemmer, E. Kondrashkina, and B.T. Nixon. Negative regulation of AAA + ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria. J. Mol. Biol. 353:242-255.

2004

Xu, H., B. Gu, B.T. Nixon, and T.R. Hoover. Purification and characterization of the AAA+ domain of Sinorhizobium meliloti DctD, a s⁵⁴-dependent transcriptional activator. J. Bacteriol. 186:3499-3507.

Xu, H., M.T. Kelly, B.T. Nixon, and T.R. Hoover. Novel substitutions in the s⁵⁴-dependent activator DctD that increase dependence on upstream activation sequences or uncouple ATP hydrolysis from transcriptional activation. Mol. Microbiol. 54:32-44.

2003

Wang, Y.-K., S. Park, B.T. Nixon, and T.R. Hoover. Nucleotide dependent conformational changes in the s⁵⁴-dependent activator DctD. J. Bacteriol. 185:6215-6219.

S.-Y. Lee, A. De La Torre, D. Yan, S. Kustu, B.T. Nixon, and D.E. Wemmer. Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA⁺ ATPase domains. Genes & Dev. 17:2552-2563.

2002

Park, S., H. Zhang, A.D. Jones, and B.T. Nixon. Biochemical evidence for multiple dimer structures for inactive vs active receiver domain of DctD. Biochemistry. 41:10934-10941.

Park, S., M. Meyer, A.D. Jones, H.P. Yennawar, N.H. Yennawar, and B.T. Nixon. Two-component signaling in the AAA + ATPase DctD: binding Mg²⁺ and BeF₃^- selects between alternate dimeric states of the receiver domain. FASEB J. 16:1964-1966.

2001

Meyer, M., S. Park, L. Zeringue, M. Staley, M. McKinstry, R.I. Kaufman, H. Zhang, D. Yan, N. Yennawar, H. Yennawar, G.K. Farber, and B.T. Nixon. A dimeric two-component receiver domain inhibits the σ54-dependent ATPase in DctD. FASEB J. 7:1326-1328.

2000

Studholme D.J., M. Buck, and B.T. Nixon. Identification of Sigma-N-dependent Promoters in Bacterial Genomes. Microbiology 146:3021-3023.

1999

Sojda, J., III, B. Gu, J. Lee, T.R. Hoover, and >B.T. Nixon. A Rhizobial Homolog of IHF Stimulates Transcription of dctA in Rhizobium leguminosarum but not in Sinorhizobium meliloti. Gene 238:489-500.

1998

Staley, M., L.C. Zeringue, R.D. Kidd, B.T. Nixon, and G.K. Farber. Crystallization and Preliminary X-ray Studies of the Rhizobium meliloti DctD Two-component Receiver Domain. Acta Cryst. D54:1416-1418.

1996

Kaufman, R.I. and B.T. Nixon. Use of PCR to Isolate Genes Encoding 54-dependent Activators from Diverse Bacteria. J. Bacteriol. 178:3967-3970.

Scholl, D. and B.T. Nixon. Cooperative Binding of DctD to the dctA UAS of Rhizobium Meliloti is Enhanced in a Constitutively Active Truncated Mutant. J. Biol. Chem. 271:26435-26442.

1994

Gu, B., J.H. Lee, T.R. Hoover, and B.T. Nixon. Rhizobium meliloti DctD, a s54-dependent Transcriptional Activator, may be Negatively Controlled by a Subdomain in the C-terminal End of its Two-component Receiver Module. Mol. Microbiol. 13:51-66.

Lee, J.H., D. Scholl, B.T. Nixon, and T.R. Hoover. Constitutive ATP Hydrolysis and Transcription Activation by a Stable, Truncated Form of Rhizobium meliloti DCTD, a s54-dependent Transcriptional Activator. J. Biol. Chem. 269:2040l-20409.

1993

Wagner, S.J., S.P. Thomas, R.I. Kaufman, B.T. Nixon, and S.E. Stevens, Jr. The glnA of the Cyanobacterium Agmenellum quadruplicatum PR-6 is Nonessential for Ammonium Assimilation. J. Bacteriol. 175:604-612.

1992

Miller, K.J., M.W. McKinstry, W.P. Hunt, and B.T. Nixon. Identification of the Diacylglycerol Kinase Structural Gene of Rhizobium meliloti 1021. Mol. Plant Microbe Interactions 5:363-371.

Ledebur, H. and B.T. Nixon. Tandem DCTD Binding Sites of the Rhizobium meliloti dctA UAS are Essential for Optimal Function Despite a 50 to 100-fold Difference in Affinity for DCTD. Mol. Microbiol. 6:3479-3492.

1991

Slatko, B.E., P.H. Heinrich, B.T. Nixon, and R.L. Eckert. Preparation of Templates for DNA Sequencing. In: Current Protocols in Molecular Biology. (Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and K. Struhl, eds), pp. 7.3.1-7.2.20. Greene Publishing Associates and Wiley-Interscience, New York, New York.

Slatko, B.E., P. Heinrich, B.T. Nixon, and D. Voytas. Construction of Nested Deletions for Use in DNA Sequencing. In: Current Protocols in Molecular Biology. (Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and K. Struhl, eds), pp. 7.2.1-7.3.9. Greene Publishing Associates and Wiley Interscience, New York, New York.